Basic studies on anti-inflammatory lipid mediators

2017 Fiscal Year Final Research Report

• Project/Area Number: 26252018
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: The University of Tokyo (2015-2017); Nagoya University (2014)
• Principal Investigator: Uchida Koji 東京大学, 大学院農学生命科学研究科(農学部), 教授 (40203533)
• Research Collaborator: SHIBATA Takahiro 名古屋大学, 大学院生命農学研究科, 准教授 (80447838)
• Project Period (FY): 2014-06-27 – 2017-03-31
• Keywords: 脂質メディエーター / プロスタグランジン / 抗炎症 / タンパク質修飾 / 神経細胞分化

Outline of Final Research Achievements

Prostaglandin D2 (PGD2) mediates important effector and regulatory functions during inflammatory response. In the present study, we studied non-enzymatic conversion of PGD2 and identified Δ12-PGD2, a structural isomer of PGD2, as one of the major albumin-dependent metabolites. The formation of Δ12-PGD2 was demonstrated in the activated RBL-2H3 mast cells. Based on the finding that the medium conditioned by activated RBL-2H3 mast cells enhanced the nerve growth factor-induced neuritogenesis of PC12 cells, we attempted to isolate an active compound from the mast cell conditioned culture medium and identified 15-deoxy-Δ12,14-PGJ2 (15d-PGJ2) as a potential enhancer of neuritogenesis. We have established a mechanism in which 15d-PGJ2-induced neuritogenesis is regulated by two sets of mechanisms, one for the translocation of a calcium channel (TRPV1) into the cell surface by NGF and one for the activation of TRPV1 by 15d-PGJ2.

Free Research Field

食糧化学