A mechanism for chromosome instability mediated by micronuclei induced with radiation

2016 Fiscal Year Final Research Report

• Project/Area Number: 26281024
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Osaka Prefecture University
• Principal Investigator: KODAMA Seiji 大阪府立大学, 理学(系)研究科(研究院), 教授 (00195744)
• Co-Investigator(Kenkyū-buntansha): 杉本 憲治 大阪府立大学, 生命環境科学研究科(系), 教授 (40196746)
• Co-Investigator(Renkei-kenkyūsha): SHIRAISHI Kazunori 大阪府立大学, 大学院理学系研究科, 助教 (40347513)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 染色体不安定化 / 放射線 / 小核形成 / ライブセルイメージング / DNA2本鎖切断

Outline of Final Research Achievements

We examined the induction of micronuclei by X-irradiation and their fate during several cell divisions post-irradiation using live cell imaging, which traced living cell lineages. Radiation promoted the induction of a whole chromosome-derived micronucleus(MN) that was mediated by chromosome segregation errors. Further, a whole chromosome-derived MN accumulated DNA double strand breaks (DSBs) during the S phase of main nuclei, and some of them were reincorporated into the main nuclei. The present study indicates the possibility that the chromosome-derived MN, which accumulates DSBs induced by radiation, is incidentally reincorporated into the main nuclei and this process causes the complicated chromosome rearrangements.

Free Research Field

放射線生物学