2016 Fiscal Year Final Research Report
Development of a tissue with in vivo regeneration capability for reconstruction of anterior cruciate ligament using decellularization and tissue-sterilization technologies
| Project/Area Number |
26282125
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biomedical engineering/Biomaterial science and engineering
|
|---|
| Research Institution | Waseda University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
加藤 義治 東京女子医科大学, 医学部, 教授 (00143850)
伊藤 匡史 東京女子医科大学, 医学部, 助教 (10328429)
坂口 勝久 早稲田大学, 理工学術院, 准教授 (70468867)
梅津 光生 早稲田大学, 理工学術院, 教授 (90132927)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 脱細胞化組織 / 膝前十字靭帯再建 / 組織再生 / 生体内組織構築 |
|---|
| Outline of Final Research Achievements |
In the treatment of knee anterior cruciate ligament (ACL) ruptured in sports and so on, patient’s own tendon is harvested and used. In this study, as a new tissue substitute, porcine tendon was used, and decellularization and sterilization technologies of tissues were established. Experiments of reconstruction of ovine knee ACL using decellularized and sterilized porcine tendons revealed that the decellularized tissues were functioned 3 months. The tissues were repopulated in vivo with ovine autologous cells and vessels were formed. Ultimate tensile loads after 3 months implantation were comparable between decellularized porcine tissues and autologous Achilles tendons. This study opened a new way to develop a novel medical device to treat ACL without need of harvesting patient’s own tendon.
|
| Free Research Field |
人工臓器学、組織工学
|
