Development of novel platform for enzyme utilization in the body based on polymeric nanocapsules

2016 Fiscal Year Final Research Report

• Project/Area Number: 26288082
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Kyushu University
• Principal Investigator: Kishimura Akihiro 九州大学, 工学(系)研究科(研究院), 准教授 (70422326)
• Research Collaborator: TANG Hengmin 九州大学, 大学院システム生命科学府 ; NAOYAMA Kenshiro 九州大学, 大学院システム生命科学府 ; YAMASAKI Hokuto 九州大学, 大学院システム生命科学府 ; TERAUCHI Mikio 九州大学, 大学院システム生命科学府 ; OGAWA Atsushi ; LIU Yiwei 九州大学, 大学院システム生命科学府
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 酵素 / ナノリアクター / ベシクル / タンパク質デリバリー / ナノ医薬品 / Biodetoxification

Outline of Final Research Achievements

In this research, a novel platform for enzyme application, which allows for expansion of its scope and durability of enzyme activity in in vivo conditions, was pioneered, particularly using polymeric hollow capsule PICsomes with a size of ~100 nm. In this project, the following three topics were mainly investigated: (1) Enzyme-based biodetoxification nanosystem, (2) Nano-delivery system for enzyme replacement therapy, (3) Nano-delivery system of light source in in vivo conditions. In the topic of (1), PICsome containing histamine degradation enzyme was developed, and successful performance was confirmed in in vivo experiments using model mice. In the topic of (2), milder methods for enzyme encapsulation was developed, and effective factor for cellular internalization of the PICsome system was revealed. In the topic (3), bioluminescent enzyme, Nano-Luc, was successfully encapsulated into PICsomes, and its activity was confirmed for future development of light-driven nanosystem.

Free Research Field

生体材料学、高分子化学、生体関連化学