Development of Immunoassay Technology with Fine Organic Chemistry and Antibody Engineering

2016 Fiscal Year Final Research Report

• Project/Area Number: 26288087
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Green/Environmental chemistry
• Research Institution: Toyohashi University of Technology
• Principal Investigator: IWASA SEIJI 豊橋技術科学大学, 工学(系)研究科(研究院), 教授 (30303712)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 残留農薬分析 / 環境分析 / 抗原抗体反応 / イムノアッセイ法 / ELISA法 / 触媒 / カルベン / イムノクロマト法

Outline of Final Research Achievements

To keep food safety and the reliance, the development of a residue analysis of low molecular agricultural chemicals is one of most important social requirements as for both human health and environmental consideration. For this regard, our objective of this research project is to develop a simple, rapid and low cost performance analytical method with immunoassay being comparable to conventional instrumental analysis such as HPLC, GC, MASS and their combination. During this research project, we have established pesticide residue analysis with immunoassay method in the range of ppb. The key technology of immunoassay is the preparation of highly selective antibody to recognize the target pesticide residue. The catalyst was newly developed to introduce suitable linker on the pesticide and also apply carbentranfer reaction of diazo-comopunds. This system is simple, rapid, cost-effective, high mobility and high throughput analytical method and now commercially available.

Free Research Field

有機合成化学