Study of GABAergic transmission mechanism using glutamate decarboxylase knockout mice

2017 Fiscal Year Final Research Report

• Project/Area Number: 26290002
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Gunma University
• Principal Investigator: YANAGAWA YUCHIO 群馬大学, 大学院医学系研究科, 教授 (90202366)
• Co-Investigator(Renkei-kenkyūsha): KAKIZAKI TOSHIKAZU 群馬大学, 大学院医学系研究科, 助教 (50375531) ; MIWA HIDEKI 群馬大学, 大学院医学系研究科, 助教 (80468488)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: GABA / GAD65 / GAD67 / ノックアウトマウス / テトラサイクリン発現システム

Outline of Final Research Achievements

GABA is a major inhibitory neurotransmitter, and it is synthesized from glutamate by glutamate decarboxylase (GAD). There are two isoforms of GAD, GAD65 and GAD67. GAD67 knockout mice died at postnatal day 0 (P0), and thus they cannot be characterized after P0. We generated time-dependent GAD67 knockout mice. In addition, we generated GAD67 knockout rats, which were found alive after 3 weeks of age. These genetically engineered rodents will be a useful tool for clarifying the role of GAD67 and GABA in brain function.

Free Research Field

神経科学