Study on the regulatory mechanism of the CAPS protein-mediated trafficking pathway of dense-core vesicles

2016 Fiscal Year Annual Research Report

• Project/Area Number: 26290026
• Research Institution: Tokyo University of Science
• Principal Investigator: 古市 貞一 東京理科大学, 理工学部, 教授 (50219094)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 有芯小胞 / CAPSタンパク質 / 開口放出 / シナプス小胞

Outline of Annual Research Achievements

CAPS1は海馬シナプスにおける放出可能なシナプス小胞のアクティブゾーンにおける安定的な状態の制御に関与する：　海馬を含む前脳特異的な条件的KO（cKO）を作製し、CAPS1の成熟海馬神経回路におけるグルタミン酸シナプスの電気生理学的解析、微細形態解析、細胞イメージング解析を実施した。その結果、CAPS1 cKOではシナプス伝達が低下し、シナプス小胞が開口放出されずにプレシナプスにたまり、開口放出が起きるアクティブゾーン近傍に分布していることがわかった。これらのことから、CAPS1は成熟海馬神経回路において、グルタミン酸シナプス伝達にも必須な機能的役割を果たすことが明らかになった。
CAPSによる有芯小胞の開口放出制御のイメージング解析：　脳内モルヒネ様ペプチド・ダイノルフィンの分泌を可視化する蛍光タンパク質を開発し、CAPS依存性の分泌を解析中である。

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Causes of Carryover

28年度が最終年度であるため、記入しない。

Expenditure Plan for Carryover Budget

28年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] The brain-specific RasGEF very-KIND is required for normal dendritic growth in cerebellar granule cells and proper motor coordination (2017)
  • Author(s): Hayashi K, Furuya A, Sakamaki Y, Akagi T, Shinoda Y, Sadakata T, Hashikawa T, Shimizu K, Minami H, Sano Y, Nakayama M, and Furuichi T.
  • Journal Title: PLoS ONE Volume: 12 Pages: e0173175
  • DOI: doi.org/10.1371/journal.pone.0173175
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Analysis of gene expression in Ca2+-dependent activator protein for secretion 2 (Cadps2) knockout cerebellum using GeneChip and KEGG pathways. (2017)
  • Author(s): Sadakata, T., Shinoda, Y., Ishizaki, Y., and Furuichi, T.
  • Journal Title: Neurosci. Lett. Volume: 639 Pages: 88-93
  • DOI: doi.org/10.1016/j.neulet.2016.12.068
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mammalian-specific central myelin protein Opalin is redundant for normal myelination: structural and behavioral assessments (2016)
  • Author(s): Yoshikawa, F., Sato, Y., Tohyama, K., Akagi, T., Furuse, T., Sadakata, T., Tanaka, M., Shinoda, Y., Hashikawa, T., Itohara, S., Sano, Y., Ghandour, SM., Wakana, S., and Furuichi, T.
  • Journal Title: PLoS ONE Volume: 11 Pages: e0166732
  • DOI: doi.org/10.1371/journal.pone.0166732
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Galacto-N-biose is neuroprotective against glutamate-induced excitotoxicity in vitro. (2016)
  • Author(s): Shinoda, Y., Nakajima, Y., Iguchi, H., Tatsumi, S., Kitaoka, M., Nakajima, M., Takahashi, T., Fujiwara, M., and Furuichi, T.
  • Journal Title: Eur. J. Pharmacol. Volume: 791 Pages: 711-717
  • DOI: doi: 10.1016/j.ejphar.2016.10.010
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] CAPS1 stabilizes the state of readily releasable synaptic vesicles to fusion competence at CA3-CA1 synapses in adult hippocampus (2016)
  • Author(s): Shinoda, Y., Ishii, C., Fukazawa, Y., Sadakata, T., Ishii, Y., Sano, Y., Iwasato, T., Itohara, S., and Furuichi, T.
  • Journal Title: Scientific Reports Volume: 6 Pages: 31540
  • DOI: doi:10.1038/srep31540
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Low doses of the mycotoxin citrinin protect cortical neurons against glutamate-induced excitotoxicity. (2016)
  • Author(s): Nakajima, Y., Iguchi, H., Kamisuki, S., Sugawara, F., Furuichi, T., Shinoda, Y.
  • Journal Title: J. Toxicol. Sci. Volume: 41 Pages: 311-319
  • DOI: doi: 10.2131/jts.41.311
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] A deficiency of Ca2+-dependent activator protein for secretion 1 affects hippocampal long-term potentiation (2016)
  • Author(s): Y. Ishii, C. Ishii, Y. Shinoda, Y. Sano, T. Furuichi
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego, CA, U.S.A.
  • Year and Date: 2016-11-12 – 2016-11-16
  • Int'l Joint Research
• [Presentation] A rubber tail task in Ca2+-dependent activator protein for secretion (CAPS2) knockout mice (2016)
  • Author(s): M. Wada, M. Ide, T. Atsumi, K. Yagishita, M., Katakai, Y., Shinoda, T. Furuichi, K.,Kansaku
  • Organizer: Society for Neuroscienc
  • Place of Presentation: San Diego, CA, U.S.A.
  • Year and Date: 2016-11-12 – 2016-11-16
  • Int'l Joint Research
• [Presentation] Enhancing Brain Transcriptome Database by Neural Gene Ontology (2016)
  • Author(s): T. Furuichi
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 神奈川県横浜市・パシフィコ横浜
  • Year and Date: 2016-07-20 – 2016-07-22
  • Invited
• [Presentation] Databasing brain gene expression information. (2016)
  • Author(s): T. Furuichi
  • Organizer: 4th INCF Japan Node International Workshop Advances in Neuroinformatics 2016 and 14th INCF Nodes Workshop
  • Place of Presentation: RIKEN Suzuki Umetaro Hall, Wako, Japan
  • Year and Date: 2016-05-28 – 2016-05-29
  • Int'l Joint Research / Invited
• [Presentation] Brain Transcriptome Database (BrainTx) (2016)
  • Author(s): A. Sato, N. Munemura, Y. Sano, Y. Shinoda, T. Sadakata, N. Morita, M. Yuzaki, T. Inoue, M. Hashimoto, H. Ohwada, H. Nishibe, Y. Yamaguchi, T. Furuichi
  • Organizer: 4th INCF Japan Node International Workshop Advances in Neuroinformatics 2016 and 14th INCF Nodes Workshop
  • Place of Presentation: RIKEN Suzuki Umemotaro Hall, Wako, Japan
  • Year and Date: 2016-05-28 – 2016-05-29
  • Int'l Joint Research
• [Presentation] Parallelization of the registration method for ISH images into the 3D standard brain atlas (2016)
  • Author(s): D. Miyammoto, H. Ikeno, Y. Okamura-Oho, Y. Okamura, N. Munemura, A. Sato, Y. Yamaguchi, R. Kanzaki, T. Furuichi.
  • Organizer: 4th INCF Japan Node International Workshop Advances in Neuroinformatics 2016 and 14th INCF Node Workshop
  • Place of Presentation: RIKEN Suzuki Umemotaro Hall, Wako, Japan
  • Year and Date: 2016-05-28 – 2016-05-29
  • Int'l Joint Research
• [Remarks] Lab of Molecular Neuroscience -Furuichi Lab
  • URL: http://www.lmn.bs.noda.tus.ac.jp/
• [Remarks] 東京理科大学理工学部応用生物科学科古市貞一
  • URL: http://www.tus.ac.jp/fac_grad/p/index.php?64da