Structural study of a huge respiratory supermolecule hemocyanin

2016 Fiscal Year Final Research Report

• Project/Area Number: 26291008
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Hokkaido University
• Principal Investigator: Tanaka Yoshikazu 北海道大学, 先端生命科学研究科(研究院), 准教授 (20374225)
• Co-Investigator(Kenkyū-buntansha): 姚 閔 北海道大学, 先端生命科学研究科(研究院), 教授 (40311518) ; 加藤 公児 北海道大学, 先端生命科学研究科(研究院), 助教 (30452428)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: X線結晶構造解析 / ヘモシアニン / 巨大蛋白質会合体 / 酸素運搬 / 糖鎖

Outline of Final Research Achievements

In this study, we have determined the X-ray crystal structure of intact hemocyanin from Japanese flying squid at 3.0 angstrom resolution. The structure urevealed the architecture of hemocyanin composed of a cylindrical wall region and five collar regions, in which the dimers of functional units (FUs) hierarchically associated to form the entire decamer. Furthermore, the roles of carbohydrates in assembly, and conserved sulfur-containing residues in copper incorporation, were revealed.

Free Research Field

構造生物化学