2017 Fiscal Year Final Research Report
Elucidation of substrate recognition mechanism of I-CLiP in the trans- and juxta-membrane regions
| Project/Area Number |
26291016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Yokohama City University |
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Principal Investigator |
NOGI Terukazu 横浜市立大学, 生命医科学研究科, 准教授 (40379102)
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 毅 大阪大学, たんぱく質研究所, 講師 (90403013)
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| Co-Investigator(Renkei-kenkyūsha) |
AKIYAMA Yoshinori 京都大学ウイルス, 再生医科学研究所, 教授 (10192460)
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| Research Collaborator |
HIZUKURI Yohei
OI Rika
TAKANUKI Kazunori
TAMURA Risako
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | タンパク質 / シグナル伝達 / 酵素 / 膜タンパク質 / 立体構造解析 |
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| Outline of Final Research Achievements |
In this project, we carried out structural and biochemical studies on intramembrane cleaving protease (I-CLiP) that catalyzes the regulated intramembrane proteolysis (RIP) so as to elucidate molecular mechanism by which I-CLiP recognizes its substrate membrane protein in the trans-membrane and juxta-membrane regions. Specifically, we studied on bacterial RsePs belonging to the site-2 protease family. It has been shown by our work that bacterial RsePs possess a characteristc intramembrane β-sheet implicated in the substrate binding. Our results contributed to a further understanding of conformational change of substrate membrane proteins within the transmembrane region.
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| Free Research Field |
構造生物学
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