Function and dynamics of stress-induced lncRNA

2017 Fiscal Year Final Research Report

• Project/Area Number: 26291018
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Ohta Kunihiro 東京大学, 大学院総合文化研究科, 教授 (90211789)
• Co-Investigator(Kenkyū-buntansha): 坪井 貴司 東京大学, 大学院総合文化研究科, 教授 (80415231)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: クロマチン / lncRNA / ヒストン修飾 / アンチセンスRNA

Outline of Final Research Achievements

We analyzed the role and dynamics of glucose-starved stress responsive long noncoding RNA (lncRNA) and antisense RNA (asRNA). We found the reciprocal expression control between lncRNA and asRNA synthesized in pairs and the histone H3K4 methylation-dependent control of asRNA expression. We observed transportation of asRNA to the cytoplasm and degradation of asRNA by nonsense-mediated degradation system. We also demonstrated that gene regulation is locally controlled by interaction between nascent lncRNA and Tup-type transcriptional repressor. In addition, we identified some lncRNAs associated with the downstream gene activation in mouse pancreatic β-cells.

Free Research Field

分子生物学