Structural basis for membrane protein integration

2016 Fiscal Year Final Research Report

• Project/Area Number: 26291023
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Tsukazaki Tomoya 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (80436716)
• Co-Investigator(Renkei-kenkyūsha): TANAKA Yoshiki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (10632333)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 蛋白質 / Sec / 膜タンパク質 / タンパク輸送 / SecYEG / X線結晶構造解析 / 膜組込み / 構造生命科学

Outline of Final Research Achievements

Crystal structures of full-length and peptide-bound SecYEG were determined. The cytoplasmic loop of SecG covers the protein-conducting channel. The cytoplasmic loop of SecG blocks protein translocation. Crystal structures of SecDF in I forms are described. The structures provide insight into an energy-coupling mechanism for Sec machinery. The structures suggest a substrate binding site and a pathway for protons.

Free Research Field

構造生命科学