Role of regions responsible for the interaction between subunits of a voltage-gated calcium channel of non-excitable cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 26291026
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: Tokyo Gakugei University
• Principal Investigator: IIDA Hidetoshi 東京学芸大学, 教育学部, 名誉教授 (70124435)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 電位作動性カルシウムチャネル / サブユニット / タンパク質間相互作用 / 出芽酵母 / 電位依存性カルシウムチャネル

Outline of Final Research Achievements

We have studied the structure and function of Cch1 and Mid1, two major subunits of a voltage-gated calcium channel homologue of non-excitable yeast cells. We have determined amino acid residues in each subunit, which are essential for the interaction between the two subunits. In addition, we have found that N-terminal 209 amino acid residues of Mid1 are not required for the function of Mid1, which is composed of 548 amino acid residues, although Mid1 has the N-terminal signal peptide composed of 20 amino acid residues.

Free Research Field

分子生物学