Development of comprehensive mapping method of target molecule linked with cell division process

2017 Fiscal Year Final Research Report

• Project/Area Number: 26291028
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: Osaka University
• Principal Investigator: IWANE Atsuko 大阪大学, 生命機能研究科, 招へい教授 (30252638)
• Co-Investigator(Kenkyū-buntansha): 渡邉 朋信 大阪大学, 免疫学フロンティア研究センター, 招へい准教授 (00375205)
• Research Collaborator: ICHINOSE Takako 大阪大学, 生命機能研究科, 招へい研究員 (40776902) ; NAGAI Rina 国立研究開発法人理化学研究所, 生命システム研究センター, テクニカルスタッフ (60392049) ; OHTA Keisuke 国立研究開発法人理化学研究所, 生命システム研究センター, 客員主管研究員 (00258401)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: FIB-SEM / 超微細構造 / 細胞分裂 / シゾン / 三次元再構築 / ダイナミックス

Outline of Final Research Achievements

In the observation of electron microscopy, FIB-SEM, whose spatial resolution does not influence the thickness of the sample theoretically, is used as a model organism of the cell division process, which is the basis of life, with a minimum of organelles Ultrastructural analysis was performed at the whole cell level while comprehensively mapping the identification of the target molecule that was not good at EM observation.
Establishment of a homologous recombinant expression system to identify multiple target molecules at the same time and to confirm the dynamics of the target molecule which is considered somewhat difficult by EM observation. Over 150 newly 3D structure models were obtained from continuous 2D image and 3D reconstruction technique. We made it is possible to obtain a more accurate ultrastructural model adapted to the time series of cell division, and the results were reported in English books.

Free Research Field

分子生物学、生物物理学、構造生物学