Analysis of the diversity of carotenoid cleavage dioxygenase genes (CCD4s) in Chrysanthemum

2016 Fiscal Year Final Research Report

• Project/Area Number: 26292014
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: The University of Tokyo
• Principal Investigator: SHIBATA Michio 東京大学, 農学生命科学研究科, 教授 (80355718)
• Co-Investigator(Renkei-kenkyūsha): OHMIYA Akemi 独立行政法人農業・食品産業技術総合研究機構, 花き研究領域, 上席研究員 (50355715)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 遺伝子 / カロテノイド / キク / バイオインフォマティクス

Outline of Final Research Achievements

The diversity of carotenoid cleavage dioxygenase genes (CCD4s)
which operates specifically in ray florets of Chrysanthemum was analyzed. Although the CCD4 genes contain minor nucleotide sequence mutations, having high sequence similarlities of more than 90% in both Chrysanthemum cultivars and wild species, overall, they showed diversity. In Chrysanthemum cultivars at least 8 kinds of CCD4 homologs existed in their genome when classified by sequence similarity of 98% or more, 6 of which were expressed in ray florets. In the diploid Chrysanthemum wild species, Chrysanthemum makinoi, CCD4 homologs similar to CmCCD4a-5 of the cultivar‘Jimba’ were expressed. CCD4 gene fragments, which have been thought not to exist in the genomes of yellow flowered cultivars and wild species, have also been found.

Free Research Field

園芸科学