2016 Fiscal Year Final Research Report
Cold temperature adaptation of hyperthermophiles
| Project/Area Number |
26292045
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kwansei Gakuin University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
跡見 晴幸 京都大学, 工学(系)研究科(研究院), 教授 (90243047)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | アーキア / 分子シャペロニン / ヘリカーゼ / 低温適応 |
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| Outline of Final Research Achievements |
Hyperthermophile is considerd as the most primitive life. Study of the adaptive mechanisms of hyperthermophiles to cold environments may provide insight into evolutionary processes. In the project, cold inducible gens in Thermococcus kodakarensis were listed and evaluated their biological functions. Cold inducible molecular chaperonin CpkA and RNA helicase Tk-DeaD play an important role for cold adaptation. Especially, a mutant chaperonin that is functional at lower temperatures enables T.kodakarensis to grow under further colder environments. Polyamine contents in cytoplasm also seem to be correlated with temperature adaptation as cytoplasmic amount of branched chain polyamine (BCPA) is decreased and that of spermidine is increased at cold stressed environment. BCPA induce the A-form in the secondary structure of DNA, while linear-PA have only a minimum effect in vitro, suggesting that temperature dependent expression is correlated with polyamine balance in cytoplasm.
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| Free Research Field |
微生物生化学
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