2016 Fiscal Year Final Research Report
The high-level usage of metabolites of dietary compounds for elucidating the molecular mechanism of functional foods
| Project/Area Number |
26292072
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Toyama Prefectural University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
室田 佳恵子 近畿大学, 理工学部, 准教授 (40294681)
榊 利之 富山県立大学, 工学部, 教授 (70293909)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ポリフェノール / 抱合代謝物 / 出芽酵母 / UDP-グルクロン-酸転移酵素 / 硫酸基転移酵素 / メチル基転移酵素 / Caco2細胞 / 共培養系 |
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| Outline of Final Research Achievements |
In order to analyze the pharmacokinetics of dietary compounds including the metabolites in the body, we have developed xenobiotic metabolizing enzymes expression systems in budding yeast. Phase II metabolites of polyphenols having variety of conjugates were synthesized by using genetically engineered budding yeast containing UDP-glucose dehydrogenase-UDP-glucuronosyltransferase, sulfotransferase or catechol methyltransferase isoforms. Pharmacokinetics of polyphenols and metabolites including absorption and metabolism into the body were examined in vitro and in vivo using standard compounds of phase II metabolites. The high-level usage of metabolites of dietary compounds would be a powerful tool for elucidating the molecular mechanism of functional foods
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| Free Research Field |
農学
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