Studies on molecular mechanism of gonadal sex differentiation and its application for aquaculture in Nile tilapia

2017 Fiscal Year Final Research Report

• Project/Area Number: 26292109
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Hokkaido University
• Principal Investigator: IJIRI SHIGEHO 北海道大学, 水産科学研究院, 准教授 (90425421)
• Co-Investigator(Kenkyū-buntansha): 小林 亨 静岡県立大学, 食品栄養科学部, 教授 (30221972) ; 足立 伸次 北海道大学, 水産科学研究院, 教授 (40231930)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: ティラピア / 性分化 / 性統御

Outline of Final Research Achievements

This study developed DNA makers which enable to select genetic male Nile tilapia. Using DNA maker, YY females were produced, resulting establishment of a method to produce all YY population. This method enable stable production of all male population as industrial useful level. In the tilapia ovarian differentiation, roles of fsh signal seemed limited and linear signaling through foxl2, cyp19a1a and estrogen production seemed sufficient signal for triggering ovarian differentiation. In the testicular differentiation, gsdf was not suggested to locate below dmrt1 signal. The fact that gsdf seemed not to affect estrogen production pathway may suggest tilapia sex differentiating factor have a role to inhibit estrogen synthesis pathway, which resulted in gsdf and dmrt1 expression that lead to trigger the testicular differentiation.

Free Research Field

魚類生殖生理学