• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2016 Fiscal Year Final Research Report

Study on genomic basis of morphogenesis in hemimetabolous insects using novel genetic modification techniques

Research Project

  • PDF
Project/Area Number 26292176
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Insect science
Research InstitutionThe University of Tokushima

Principal Investigator

Mito Taro  徳島大学, 生物資源学部, 准教授 (80322254)

Co-Investigator(Kenkyū-buntansha) 友成 さゆり  徳島大学, ソシオテクノサイエンス研究部, 技術専門職員 (40448345)
渡辺 崇人  徳島大学, 農工商連携センター, 特任助教 (30709481)
Project Period (FY) 2014-04-01 – 2017-03-31
Keywordsゲノム編集 / 昆虫 / 胚発生 / 後胚発生
Outline of Final Research Achievements

We established a technical basis for in vivo analysis of expression patterns and functional analysis of genes regulating embryogenesis in Gryllus bimaculatus, a hemimetabolous insect model system. Using CRISPR/Cas9 system, we developed a gene knock-in technique in the cricket. We applied this technique to knock-in/knockout for efficient identification of knock-out individuals by detecting marker gene expression. In addition, we succeeded in observation of gene expression patterns through developmental stages via marker gene knock-in. Data of expression and function of Hox genes was obtained, including spatial expression patterns in postembryonic stages. Furthermore, we showed availability of our knock-in method to enhancer trap analysis.

Free Research Field

発生生物学・昆虫科学

URL: 

Published: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi