Development of therapeutic systems based on three-dimensional DNA nanostructures

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293008
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Kyoto University
• Principal Investigator: Nishikawa Makiya 京都大学, 薬学研究科(研究院), 准教授 (40273437)
• Co-Investigator(Kenkyū-buntansha): 高橋 有己 京都大学, 薬学研究科(研究院), 助教 (00547870) ; 高倉 喜信 京都大学, 薬学研究科(研究院), 教授 (30171432)
• Co-Investigator(Renkei-kenkyūsha): ENDO Masayuki 京都大学, 物質-細胞統合システム拠点, 准教授 (70335389) ; WATANABE Hiroshi 京都大学, 化学研究所, 教授 (90167164)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 核酸 / DNA / DDS / ハイドロゲル / 抗原

Outline of Final Research Achievements

To develop therapeutic systems based on three-dimensional DNA nanostructures, we newly designed several types of basic DNA units. The comparison of their properties revealed that tetrapod-like structured DNA was the DNA nanostructure with the highest formation efficiency and uptake by immune cells. Then, a self-assembling gelling system was developed to obtain DNA hydrogel. Rheological analysis revealed that this hydrogel is injectable because of its quick gel-sol transition, and is regelated after injection. We also found that the antitumor effects of antigen-loaded DNA hydrogel can be greatly improved by the use of cationized antigen, which was more slowly released from the hydrogel than unmodified antigen.

Free Research Field

生物薬剤学