Elucidation of transcriptional regulation by histone modification pattern

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293059
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Tohoku University
• Principal Investigator: NAKAYAMA Keiko 東北大学, 医学(系)研究科(研究院), 教授 (60294972)
• Co-Investigator(Kenkyū-buntansha): 舟山 亮 東北大学, 医学(系)研究科(研究院), 助教 (20452295) ; 細金 正樹 東北大学, 医学(系)研究科(研究院), 助手 (30734347)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ヒストン修飾 / Ｂリンパ球 / ChIPシークエンス / 乳癌細胞株

Outline of Final Research Achievements

TGF-β treatment to breast cancer cell lines induces epithelial-mesenchymal transition. We confirmed suppression of EZH2 expression by shRNA decreased modification levels of Histone H3K27me3. With this method, we analyzed the effect of suppression of H3K27me3 modification on transcript. Contrary to expectations, suppression of H3K27me3 did not cause a major effect.
Next to breast cancer cell lines, we analyzed EZH2 deficient B lymphocytes. As we expected, modification of H3K27me3 in these downregulated and we obtained list of transcriptional activated genes. We also analyzed the modification level of H3K4me1 as a marker of enhancer activity. We identified the region where H3K4me1 increased after EZH2 deletion. However, we could not recognize which genes were regulated by corresponding enhancers.

Free Research Field

エピゲノム・H3K27me3 ・ シークエンス