2016 Fiscal Year Final Research Report
Role of APOBEC deaminases in human tumor virus infection
| Project/Area Number |
26293103
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KITAMURA Kouichi 金沢大学, 医学系, 助教 (70378892)
WAKAE Kousho 金沢大学, 医学系, 助教 (70638303)
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| Research Collaborator |
KOURA Miki
SIMADU Miyuki
Que Lusheng
Li Yingfang
Mohiuddin Md
Liu Guangyan
Monjurul Ahasan
Wang Zhe
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | APOBEC3 / AID / virus / hypermutation / Hepatitis B virus / Papillomavirus |
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| Outline of Final Research Achievements |
We investigated antiviral role of immune effecter molecules APOBEC in viral infection of papillomavirus (HPV) and hepatitis B virus (HBV). Experimental results suggest that APOBECs reduce infectivity of HPV16 pseudovirion by interfering viral assemble in vitro. DNA sequencing of clinical samples derived from precancerous cervixs revealed that APOBECs introduce hypermutation in HPV16 viral DNA. Mapping of mutation identifies viral promoter and replication origin as a hot spot of hypermutation mechanism. We also found that AID is induced in hepatocytes by TGFb stimulation and AID forms an RNP complex of viral P protein and host RNA exosome, resulting in degradation of HBV RNA. These studies demonstrate significant role of APOBEC protein in HPV and HBV infection.
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| Free Research Field |
virus
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