Development of an innovative lymphocyte chip to establish personalized immuno-therapy for infectious diseases

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293237
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: University of Toyama
• Principal Investigator: MURAGUCHI Atsushi 富山大学, 大学院医学薬学研究部(医学), 教授 (20174287)
• Co-Investigator(Kenkyū-buntansha): 岸 裕幸 富山大学, 大学院医学薬学研究部(医学), 准教授 (60186210) ; 小澤 龍彦 富山大学, 大学院医学薬学研究部(医学), 助教 (10432105) ; 浜名 洋 富山大学, 大学院医学薬学研究部(医学), 助教 (90551549) ; 小林 栄治 富山大学, 大学院医学薬学研究部(医学), 助教 (70459733)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: リンパ球チップ / Tリンパ球 / T細胞受容体 / 免疫治療 / 個の免疫

Outline of Final Research Achievements

To develop an innovative lymphocyte chip to establish personalized immuno-therapy for infectious diseases, 1) We developed a new lymphocyte chip using microwave-coated chip that were able to efficiency trap single microwave-beads coated lymphocytes. 2) We succeeded in retrieving a single antigen-specific T lymphocytes from lymphocyte chip and efficiently analyzing TCR gene sequences of alpha- and beta-TCR gene. 3) We succeeded in detection of antigen-specific CD4-positive T lymphocytes using lymphocyte chip. 4) We improved a method for verifying TCR function using various target cell lines. 5) We successfully constructed sTCR ( a dimer containing TCR V-region and TCR C-region) and scFv-TCR ( a monomer containing V-alpha gene and V-beta gene in a single polypeptide). 6) We established 4 kinds of human anti-West Nile virus monoclonal antibodies with neutralizing activities.

Free Research Field

免疫学