Molecular mechanism of stem cell leukemogenesis and regulation of hematopoietic cell growth

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293247
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Mie University
• Principal Investigator: Nosaka Tetsuya 三重大学, 医学(系)研究科(研究院), 教授 (30218309)
• Co-Investigator(Renkei-kenkyūsha): ONO RYOICHI 三重大学, 大学院医学系研究科, 講師 (40422414)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: PLZF / 白血病幹細胞 / 白血病モデルマウス / EYA2

Outline of Final Research Achievements

We found that PLZF plays a pivotal role in hematopoietic stem cell leukemogenesis by using transgenic mice conditionally expressing an MLL chimeric gene. EYA2 gene expression was found to be elevated downstream of PLZF, and EYA2 expression was sufficient to immortalize hematopoietic cells. Furthermore, EYA2 knockdown resulted in reduced growth of the hematopoietic cells immortalized by PLZF or PLZF-RARA. These results suggest that the PLZF-EYA2 axis is important in leukemogenesis.

Free Research Field

血液学、分子生物学