Development of comprehensive cellular therapy for acute and late-onset radiation-induced injuries

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293277
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Hiroshima University
• Principal Investigator: Tatsuo Ichinohe 広島大学, 原爆放射線医科学研究所, 教授 (80314219)
• Co-Investigator(Kenkyū-buntansha): 三浦 康生 京都大学, 医学(系)研究科(研究院), 助教 (70605146) ; 前川 平 京都大学, 医学(系)研究科(研究院), 教授 (80229286)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 被ばく医療 / 組織再生治療 / 造血細胞移植 / 間葉系幹細胞

Outline of Final Research Achievements

In this study, we aimed to develop comprehensive cellular therapy for acute syndrome and late effects associated with radiation-induced tissue/organ injuries by use of differential regenerative functions of hematopoietic stem cells (HSCs) and mesenchymal stem/stromal cells (MSCs). We found that dual transplantation of HSCs and MSCs ameliorates skin and large bowel injuries associated with acute radiation exposure and graft-versus-host disease compared with transplant of HSCs alone in an irradiated (8 Gy) mouse model. Intriguingly, such protective effect was reproducible in mice receiving HSCs with culture supernatant of MSCs instead of MSCs themselves. We also established a unique method to efficiently isolate MSCs from human umbilical cord blood for the future MSC banking system free from invasive procedures to the donors .

Free Research Field

造血幹細胞移植　細胞治療