The attempt of new molecular therapy for esophageal cancer using the analysis of regulation concerning genome and epigenome

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293298
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Chiba University
• Principal Investigator: Matsubara Hisahiro 千葉大学, 医学(系)研究科(研究院), 教授 (20282486)
• Co-Investigator(Kenkyū-buntansha): 井ノ上 逸朗 国立遺伝学研究所, 総合遺伝研究系, 教授 (00192500) ; 阿久津 泰典 千葉大学, 医学(系)研究科(研究院), 講師 (00375677) ; 村上 健太郎 千葉大学, 医学部附属病院, 助教 (40436382) ; 田村 裕 千葉大学, 医学(系)研究科(研究院), 准教授 (50263174)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 食道癌 / ゲノム / エピゲノム / 分子治療

Outline of Final Research Achievements

Analysis of allele specific expression in esophageal squamous cell carcinoma with combination of exome sequencing and mRNA Sequencing. Exome sequenceing analysis of 25 ESCC cases identified TP53 and ZNF750 as significantly mutated genes. Each gene was analyzed in detail with combination of DNA sequencing data and RNA sequencing. The expression level of TP53 with splicing site mutations, stopgain mutations, and indels were decreased significantly, which was seemed to cause dysfanction of p53 protein. Although expression of TP53 with nonsynchronous mutation was maintained, frequencies of nonsynchronous mutation allele in RNA were increased than those in DNA, which possibly cause dysfanction of its product. Comparing between normal and tumor tissues, expression of VNF750 were decreased, which ZNF750 expression of 2 cases with nonsynchronous mutations were maintained.

Free Research Field

消化器外科