2017 Fiscal Year Final Research Report
Development of versatile fractionation method of human atrial myocytes from Tet-1 stabilized-human iPS cells
| Project/Area Number |
26293313
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
井口 篤志 埼玉医科大学, 医学部, 教授 (90222851)
加藤 英政 愛媛大学, 医学(系)研究科(研究院), 准教授 (50292123)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | iPS細胞 / 心不全 / 再生 |
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| Outline of Final Research Achievements |
For development of a versatile human induced pluripotent stem cells (hiPSc) method, HiPSc induction was performed with patient’s primary somatic cells that are fibroblasts from the skin and the heart. However, induction efficacy of hiPSc was very low compared with that of commercial available human fibroblasts. The fibroblasts from the remainder tissue after surgery highly expressedα－SMA（smooth muscle actin）, so-called these are myofibroblats. Although using the myofibroblasts, it was very hard to create hiPSc, an initial medium without TGF-βfor hiPSc induction led to development of hiPSc successfully. But it was still low efficacy. Using the hiPSc from the myofibroblasts, we achieved the cardiomyocytes differentiation.
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| Free Research Field |
心不全
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