Fluorescence imaging for investigation and treatment of bone and soft tissue tumors

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293333
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kanazawa University
• Principal Investigator: Tsuchiya Hiroyuki 金沢大学, 医学系, 教授 (40227434)
• Co-Investigator(Kenkyū-buntansha): 林 克洋 金沢大学, 大学病院, 助教 (80507054) ; 山本 憲男 金沢大学, 医薬保健学総合研究科, 教授 (90332668) ; 三輪 真嗣 金沢大学, 大学病院, 医員 (40753455) ; 木村 浩明 名古屋市立大学, 医学(系)研究科(研究院), 助教 (50608693)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 骨軟部腫瘍 / 転移 / 新規治療 / 転移メカニズム

Outline of Final Research Achievements

We demonstrate in vivo fluorescence imaging of bone-marrow micrometastasis of prostate cancer at the cellular level in nude mice using PC-3 human prostate cancer cells labeled with green fluorescent protein (GFP). PC-3-GFP cells were visualized by real-time fluorescence imaging, to traffic and grow in the bone marrow. Formation of bone marrow micrometastasis could be imaged at the single-cell level in live mice, using confocal microscopy.
We investigated the efficacies of fluorescence-guided surgery (FGS), genetically-modified auxotrophic strain of Salmonella typhimurium (A1-R), and glycogen synthase kinase-3β (GSK- 3β) inhibitors. FGS significantly reduced residual tumor as well as the recurrence of experimental prostate-cancer bone metastasis. A1-R significantly inhibited tumor growth and metastasis in soft tissue tumor model and bone metastasis model. Treatment with GSK-3β inhibitors attenuated the growth of orthotopic osteosarcoma in mice.

Free Research Field

整形外科学