Identification of pathogens in sewage collected in Spain in order to predict imported infectious agents in Japan

2016 Fiscal Year Final Research Report

• Project/Area Number: 26303011
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Civil and environmental engineering
• Research Institution: Hokkaido University
• Principal Investigator: Sano Daisuke 北海道大学, 工学(系)研究科(研究院), 准教授 (80550368)
• Co-Investigator(Kenkyū-buntansha): 大村 達夫 東北大学, 学内共同利用施設等, 教授 (30111248) ; 片山 浩之 東京大学, 工学(系)研究科(研究院), 准教授 (00302779) ; 石井 聡 北海道大学, 工学(系)研究科(研究院), 助教 (10612674)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 病原体 / 下水 / スペイン

Outline of Final Research Achievements

This study aims to identify pathogens in wastewater collected in Spain. Firstly, a simultaneous detection method of pathogenic viruses and bacteria was developed. Wastewater samples and fecal samples from gastroenteritis patients were collected in Spain, and applied to the newly developed method. As a result, norovirus GII was found to be the most important infectious agent in wastewater. Norovirus genome sequences were then analyzed by next-generation sequencing, and its nucleotide diversity was evaluated. Also, primer and probe sets for separately quantifying multiple genotypes of norovirus were designed and validated.

Free Research Field

土木環境システム