Development of Early Diagnosis System for Alzheimer's Disease by Electrochemical Detection of Amyloid beta peptide

2016 Fiscal Year Final Research Report

• Project/Area Number: 26350552
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical systems
• Research Institution: Konan University
• Principal Investigator: FUJII Satoshi 甲南大学, フロンティアサイエンス学部, 教授 (80271518)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: アルツハイマー病 / アミロイドβ / 電気化学センサー / 早期診断 / バイオセンサー / ペプチド

Outline of Final Research Achievements

We have developed an electrochemical sensor that aspires for early diagnosis of Alzheimer’s disease by quantifying plasma amyloid-beta peptide. Amyloid-beta (Abeta) fibrillization promoting peptide, AFPP, which we have developed, was immobilized on a gold electrode, and then Abeta was added. After accumulation of Abeta on AFPP, cupper ion was added and electrochemical measurements were carried out. By using this procedure, we succeeded in measuring Abeta with the limit of detection, 5 nM. To improve the sensitivity, we made a golod nanoparticle modified electrode. Although the sensitivity was improved, the physical strength of the modified electrode became worth. Therefore, there is a problem with reusability after initialization by washing. Currently the sensitivity of our method does not reach the plasma Abeta level. Further investigations are now under way.

Free Research Field

生物無機化学の医療応用