2016 Fiscal Year Final Research Report
Development of analysis technique for accelerating protein aggregation and crystallization under applied electric fields and the clarification of the mechanism
Project/Area Number |
26390049
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied materials
|
Research Institution | Fukushima National College of Technology (2016) Ibaraki National College of Technology (2014-2015) |
Principal Investigator |
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Keywords | タンパク質 / 凝集体 / 結晶化 / 光散乱 / 電場刺激 / フラクタル / 微粒子 |
Outline of Final Research Achievements |
We have developed a new analysis apparatus with the ability to in situ measure forward scattering light at small angle for crystallizing protein solutions under a low applied voltage. We tracked protein lysozyme aggregation in the pre-crystalline solutions using the developed apparatus. An enhancement of forward scattering intensities was observed for lysozyme solutions with a low-concentration salt under electric fields, which were under not crystallizing condition without applied voltages. We have found that the application of electric fields provides an effect of accelerating a formation of fractal aggregates on the pre-crystalline step.
|
Free Research Field |
応用物性
|