2016 Fiscal Year Final Research Report
Development of analysis technique for accelerating protein aggregation and crystallization under applied electric fields and the clarification of the mechanism
| Project/Area Number |
26390049
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied materials
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| Research Institution | Fukushima National College of Technology (2016)
Ibaraki National College of Technology (2014-2015) |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | タンパク質 / 凝集体 / 結晶化 / 光散乱 / 電場刺激 / フラクタル / 微粒子 |
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| Outline of Final Research Achievements |
We have developed a new analysis apparatus with the ability to in situ measure forward scattering light at small angle for crystallizing protein solutions under a low applied voltage.
We tracked protein lysozyme aggregation in the pre-crystalline solutions using the developed apparatus. An enhancement of forward scattering intensities was observed for lysozyme solutions with a low-concentration salt under electric fields, which were under not crystallizing condition without applied voltages. We have found that the application of electric fields provides an effect of accelerating a formation of fractal aggregates on the pre-crystalline step.
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| Free Research Field |
応用物性
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