Analysis of DNA strand breaks induced by exposure to an atmospheric pressure plasma jet

2016 Fiscal Year Final Research Report

• Project/Area Number: 26390096
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plasma electronics
• Research Institution: Toyohashi University of Technology
• Principal Investigator: Kurita Hirofumi 豊橋技術科学大学, 工学(系)研究科(研究院), 助教 (70512177)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: プラズマ医療応用 / 大気圧低温プラズマ / DNA損傷

Outline of Final Research Achievements

The use of a molecular beacon for rapid detection of DNA strand breaks induced by atmospheric pressure plasma jet (APPJ) irradiation was demonstrated. MBs are oligonucleotides that adopt a stem-and-loop structure and carry a 5’-fluorescent moiety and a 3’-nonfluorescent quenching moiety. Scission of the molecular beacon by APPJ irradiation leads to separation of the fluorophore-quencher pair, resulting in an increase in fluorescence that directly correlates with the DNA strand breaks. The results show that the increase in fluorescence intensity is proportional to the exposure time and the rate of fluorescence increase is proportional to the discharge power. In addition, This simple and rapid method allows the estimation of the extent of DNA stand breaks induced by exposure to a non-thermal plasma. In addition, it was shown that the plasma jet readily induced DNA-strand breaks in the cell model, surprisingly without any significant poration or rupture of the phospholipid membrane.

Free Research Field

プラズマエレクトロニクス