Study on function inhibitory effect of protein by time-resolved fluorescence measurement

2016 Fiscal Year Final Research Report

• Project/Area Number: 26410029
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical chemistry
• Research Institution: Institute for Laser Technology
• Principal Investigator: TANIGUCHI SEIJI 公益財団法人レーザー技術総合研究所, レーザーバイオ化学研究チーム, 副主任研究員 (00342725)
• Co-Investigator(Renkei-kenkyūsha): CHOSROWJAN Haik 公益財団法人レーザー技術総合研究所, レーザーバイオ化学研究チーム, 副主任研究員 (70291036) ; KITAMURA Masaya 大阪市立大学, 大学院・工学研究科, 教授 (20244634) ; NAKANISHI Takeshi 大阪市立大学, 大学院・工学研究科, 講師 (20422074)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 分子分光 / 時間分解蛍光 / 蛋白質・酵素 / 機能阻害効果

Outline of Final Research Achievements

To study the function inhibitory effect of the protein (enzyme), we investigated the change of the photo-excitation processes with or without inhibitory molecules using serine hydroxyl transferase (SHMT) and D-amino acid oxidase (DAAO) by time-resolved fluorescence measurements. In SHMT, it was revealed that fluorescence quenching process at the addition of amino acids such as L-serine and glycine was caused by external aldimine formation and this reaction was accelerated by photo-excitation. For DAAO, it was clarified for the first time that the dimer present in aqueous solution is formed by subunits with different protein structures, and the effect by inhibitor also differs in each subunit. From these, it was shown that the time-resolved fluorescence method is useful for measuring the inhibitory effect.

Free Research Field

物理化学