2016 Fiscal Year Final Research Report
Improvemtnt of reactive pyrolysis GC/MS system for highly sensitive detection of drug-resistant bacteria
| Project/Area Number |
26410164
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Chubu University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 消毒薬耐性菌 |
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| Outline of Final Research Achievements |
A reactive pyrolysis GC/MS system combined with magnetic bead probe was newly developed. This technique realized 1) selective capture of a certain species of bacteria, and 2 ) fatty acid analysis of the species. Moreover, this method was applied to the analysis of fatty acid components in mutant-type E. coli with resistance to 5-chloro-2-(2,4-dichlorophenoxyl)phenol (triclosan). Based on the pattern of the obtained chromatograms, the mutant colonies were clearly discriminated from the wild type ones reflecting their resistance to triclosan.
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| Free Research Field |
分析化学
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