Tailor-made artificial enzymes (catalytic antibodies) by the development of holo-abzymes

2016 Fiscal Year Final Research Report

• Project/Area Number: 26410184
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Osaka Prefecture University
• Principal Investigator: Tsumuraya Takeshi 大阪府立大学, 理学(系)研究科(研究院), 准教授 (00372855)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 酵素 / モノクローナル抗体 / 抗体酵素 / 免疫学 / タンパク質 / 分子認識 / 生体分子 / 人工酵素

Outline of Final Research Achievements

In this work we have developed holo-abzyme in which the antigen-combining site can bind the artificial cofactors which have functional groups　for the catalysis. The holo-abzyme catalyzes various types of chemical transformations by simply exchanging the artificial cofactors. We also succeeded in the X-ray crystal analysis of holo-abzyme 27C1, showing the molecular basis of the catalysis by 27C1.

Free Research Field

生物有機化学