Molecular function of the BDNF noncoding RNA

2016 Fiscal Year Final Research Report

• Project/Area Number: 26430032
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Kojima Masami 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 研究特別チーム長 (40344171)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 神経細胞 / RNA / 翻訳後メカニズム / タンパク質 / マウス / 情動障害

Outline of Final Research Achievements

BDNF-processing deficit mice expressed proBDNF protein with a little amount of BDNF, and a pronouced behavioral phenotype includes depression-like behavior. In this study, the gene expression of BDNF was analyzed to find the molecular pathology of this abnormal mouse. First, we found that novel BDNF antisense RNA is expressed in the hippocampal region of this mouse. In order to find out the function, the antisense RNA was expressed in cultured neurons and we found that BDNF antisense RNA named AS2c had the role of increasing BDNF protein. At the same time as applying for a patent for this result, we are currently preparing a manuscript for submission of the article.

Free Research Field

神経科学、分子生物学