2016 Fiscal Year Final Research Report
Molecular basis of lifespan and aging by exposing LED light in C. elegans
| Project/Area Number |
26430068
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Neurochemistry/Neuropharmacology
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | C. elegans / LED light |
|---|
| Outline of Final Research Achievements |
To invesigate the biological disadvantage of blue LED light, we used the free-living nematode Caenorhabditis elegans, as a model. By exposing blue LED light at 465nm, the lifespan of C. elegans was decreased, and these reactive oxygen species (ROS) activity as a marker of stress response were slightly increased. Using Real-Time RT-PCR analysis to measure gene expression levels by exposure of LED light, we found that mec-5 gene which express in sensory neuron was highly expressed and neural calcium sensor (ncs-1) gene was significantly decreased in worms with blue LED light as compared to dark exposure as a control. Furthermore, we found to cause a marked deterioration in the worm's behavior with blue LED light. It is concluded that exposure of blue LED light may cause oxidative stress, and result in abnormal locomotion and survival in the nematode C. elegans. However, further investigation is required to clarify the toxicological effect of the LEDs light.
|
| Free Research Field |
分子生物学
|
