Functional analysis of amino acid transporter LAT1 in tumor endothelial cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26430121
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: Kyorin University
• Principal Investigator: Suehiro Jun-ichi 杏林大学, 医学部, 助教 (80572601)
• Co-Investigator(Renkei-kenkyūsha): WADA Youichiro 東京大学, アイソトープ総合センター, 教授 (10322033) ; SAKURAI Hiroyuki 杏林大学, 医学部, 教授 (00508294)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: がん微小環境 / アミノ酸トランスポーター / 腫瘍血管新生

Outline of Final Research Achievements

To elucidate the pathological roles of system L amino acid transporter LAT1 in tumor microenvironment, we examined in vitro endothelial function and in vivo tumor angiogenesis using LAT1 selective inhibitor, JPH203. JPH203 elicited amino acid deprivation stress and impaired endothelial cell growth, migration and tube formation in human umbilical vein endothelial cells. In mouse B16F10 melanoma xenograft model, JPH203 significantly inhibited tumor growth by attenuating angiogenesis. Taken together, these findings have provided the new insight in LAT1 function in tumor progression and showed the possibility of JPH203 as an angiogenesis inhibitor.

Free Research Field

血管生物学