Fundamental study of niche modification for reducing cancer stem cell properties

2017 Fiscal Year Final Research Report

• Project/Area Number: 26430125
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: Kyoto Sangyo University
• Principal Investigator: ITANO Naoki 京都産業大学, 総合生命科学部, 教授 (40257712)
• Research Collaborator: IZUMIKAWA Tomomi 京都産業大学, 総合生命科学部, 研究助教 ; Chanmee Theerawut 京都産業大学, 総合生命科学部, 研究員 ; Ontong Pawared 京都産業大学, 大学院・工学研究科, 学生 ; MOCHIZUKI Nobutoshi 京都産業大学, 大学院・生命科学研究科, 学生 ; HIGASHIDE Miho 京都産業大学, 大学院・生命科学研究科, 学生 ; Chokchaitaweesuk Chatchadawarai 京都産業大学, 大学院・生命科学研究科, 学生
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: がん幹細胞 / ニッチ / ストレス耐性 / ヒアルロン酸

Outline of Final Research Achievements

CSCs are thought to be responsible for tumor recurrence, due to their resistance to chemo- and radiotherapy. CSCs reside in a special microenvironmental niche that provides a favorable microenvironment for their self-renewal and maintenance. We have previously demonstrated that overproduction of hyaluronan (HA), a primary component of stem cell niche, promotes acquisition of CSC signatures. Here, we investigated the metabolic reprogramming in HA-overproducing cells by stable isotope-assisted tracing and mass spectrometry profiling. These integrated approaches disclosed an acceleration of metabolic flux in the hexosamine biosynthetic pathway (HBP). Overexpression or gene silencing of glutamine:fructose-6-phosphate amidotransferase 1, an HBP rate-limiting enzyme, suggests that HA production regulates drug resistance-related properties of CSCs via HBP.

Free Research Field

分子腫瘍学