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2016 Fiscal Year Final Research Report

Functional analysis of Peg10, a paternally expressed imprinted gene

Research Project

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Project/Area Number 26430183
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Genome biology
Research InstitutionNational Institute of Health Sciences

Principal Investigator

Ono Ryuichi  国立医薬品食品衛生研究所, 毒性部, 室長 (10401358)

Project Period (FY) 2014-04-01 – 2017-03-31
Keywords胎盤 / レトロトランスポゾン
Outline of Final Research Achievements

We have produced Peg10 mutant mice by introducing DSB using CRISPR/Cas9 system to analyze detailed function of Peg10. Then, we found that about 30 % of mutant mice had long de novo retrotransposon insertion(s) at DSB-introduced sites. In other cases, mutant mice had insertions from spliced mRNAs , indicating the involvement of reverse transcription (RT).In conclusion, RT-product, in the mouse zygote, were captured at DSB loci mediated by micro homology.

Free Research Field

エピジェネティクス

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Published: 2018-03-22  

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