2016 Fiscal Year Final Research Report
Functional analysis of Peg10, a paternally expressed imprinted gene
| Project/Area Number |
26430183
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
Ono Ryuichi 国立医薬品食品衛生研究所, 毒性部, 室長 (10401358)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 胎盤 / レトロトランスポゾン |
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| Outline of Final Research Achievements |
We have produced Peg10 mutant mice by introducing DSB using CRISPR/Cas9 system to analyze detailed function of Peg10. Then, we found that about 30 % of mutant mice had long de novo retrotransposon insertion(s) at DSB-introduced sites. In other cases, mutant mice had insertions from spliced mRNAs , indicating the involvement of reverse transcription (RT).In conclusion, RT-product, in the mouse zygote, were captured at DSB loci mediated by micro homology.
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| Free Research Field |
エピジェネティクス
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