Regulation of peroxisome biogenesis by protein phosphorylation and signal transduction

2016 Fiscal Year Final Research Report

• Project/Area Number: 26440032
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyushu University
• Principal Investigator: Okumoto Kanji 九州大学, 理学研究院, 助教 (20363319)
• Co-Investigator(Renkei-kenkyūsha): FUJIKI Yukio 九州大学, 生体防御医学研究所, 特任教授 (70261237)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ペルオキシソーム / リン酸化 / タンパク質輸送 / 一次繊毛 / 繊毛病

Outline of Final Research Achievements

A peroxisomal membrane protein Pex14p plays a pivotal role in peroxisomal matrix protein import. In this study, we found that Pex14p is phosphorylated upon oxidative stresses. Phosphorylation of Pex14p regulates import of peroxisomal matrix proteins, which can counteract cellular oxidative stresses. We also found that NDR2, a protein kinase involved in primary cilium formation, partially localized to peroxisomes in a manner dependent on its peroxisome-targeting signal-1-like sequence, Gly-Lys-Leu, at the C-terminus. Novel peroxisomal function is implicated in ciliogenesis and pathogenesis of ciliopathies.

Free Research Field

分子細胞生物学