2016 Fiscal Year Final Research Report
Thermal adaptation mechanism of RNA bacteriophage Qb
Project/Area Number |
26440194
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Evolutionary biology
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Research Institution | Hirosaki University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Keywords | 適応進化 / RNAバクテリオファージ / 大腸菌 |
Outline of Final Research Achievements |
In the evolutionary biology, it is very important issue to identify genes and mutations that are responsible for the adaptation. We performed a thermal adaptation experiment using the single-stranded RNA bacteriophage Qβ in which the culture temperature was increased from 37.2℃ to an inhibitory temperature of 43.6℃ in a stepwise manner in three independent lines. And we obtained three kinds of mutants that were able to amplify at 43.6℃. In this study, we measured their fitness and infection cycle quantitatively, and identified the responsible genes and substitutions for thermal adaptation of Qβ.
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Free Research Field |
実験進化学
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