Identification of S genes of Ipomoea trifida, a wild relative of sweetpotato

2017 Fiscal Year Final Research Report

• Project/Area Number: 26450002
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Mie University
• Principal Investigator: Tsuchiya Tohru 三重大学, 地域イノベーション推進機構, 准教授 (30293806)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 自家不和合性 / サツマイモ野生種 / 自他認識 / S遺伝子

Outline of Final Research Achievements

Ipomoea trifida have sporophytic self-incompatibility system. In I.trifida, AB2 gene were identified as male S-candidate gene, SE2 and SEA genes were identified as female S-candidate genes. To determine male S-gene of I.trifida, S1-AB2 peptides were chemically synthesized and applied to S1 homozygous stigma before pollination with S3 pollen. According to this bioassay, it is strongly suggested that AB2 may act as male S-gene, because pollinated S3 pollen were not germinated on the S1 stigma. To determine female S-gene of I.trifida, S1-S candidate genes were introduced into S10 homozygous I.trifida, however, S-gene was not determined by this experiment because of low fertility of transgenic pollen grains. To determine S-genes of I.trifida, comparative analysis were carried out with S-loci sequences from 4 self-incompatible I.trifida lines and 2 self-compatible I.trifida and I.nil lines. From this analysis, AB2 and SEA genes were identified as predominant genes for S-genes in I.trifida.

Free Research Field

植物分子育種蕚