2016 Fiscal Year Final Research Report
Identification of genes regulating seed size in rice
| Project/Area Number |
26450008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Fukui Prefectural University |
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Principal Investigator |
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| Research Collaborator |
MIURA Kotaro 福井県立大学, 生物資源学部, 准教授 (70571561)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | イネ / 種子形 / 粒形変異体 / ３量体Gタンパク質 / ブラシノステロイド |
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| Outline of Final Research Achievements |
In order to understand mechanism regulating seed size, the identification and isolation of genes regulating seed size using novel seed size mutants in rice, srs2 and srs6 were carried out. A SNP due to nonsense mutation was detected in an orthologue gene to Arabidopsis BRASSINOSTEROID-SIGNALING KINASE 2 (BSK2) in srs2 mutant. A one base substitution due to generate a stop codon was found ERECT PANICLE 3 (EP3) gene, which encodes F-box protein in srs6 mutant.
Now we start the complementation test introducing wild type genes and produce the knock out rice using CRISPR-Cas 9 system, in order to proof the function of the candidate genes.
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| Free Research Field |
植物生化学
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