Identification of petal-specific cis-element and its application to create a novel 'blue tulip'.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450049
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: Toyama Prefectural Agricultural
• Principal Investigator: Shoji Kazuaki 富山県農林水産総合技術センター, 富山県農林水産総合技術センター農業研究所, 副主幹研究員 (50504461)
• Research Collaborator: YAMADA Kyoji ; WAKASUGI Tatsuya ; MARUYAMA Atsuko ; MIZUSIMA Miu ; ANDO Takako ; IKEGAWA Shiho
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: チューリップ / ユリ / プロモーター / シスエレメント / 花色 / 形質転換

Outline of Final Research Achievements

Flower petal-specific cis-elements of MYB genes, which are expressing only at the petals, were identified in tulip and lily plants. Analysis was done by original method using a combination with introducing mutations in MYB promoter regions and vacuolar iron transporter (VIT) as a reporter gene. After bombardment to purple or red petal cells, we could decide the cis-element sequences by altering the color or not. If mutation is not introduced in cis-element region, the color will change to blue. But, if mutation is introduce in its region, the color will never change. As the results, three TCAT motifs in tulip and AAACTTT motif in lily were suggested as cis-elements. Quantitative analysis of motifs using luciferase (Luc) as a reporter gene was also supported these sequences.
In this study, we could obtain the transgenic plants from tulip by super agrobacterium method using a vector harboring these promoters and VIT gene for produce a novel 'blue tulip'.

Free Research Field

植物分子生物学