Production and purification of fatty acid contributing to prevention of atopic dermatitis through microbial conversion and enzymatic process.

2017 Fiscal Year Final Research Report

• Project/Area Number: 26450113
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Osaka Research Institute of Industrial Science and Technology (2017); Osaka Municipal Technical Research Institute (2014-2016)
• Principal Investigator: Nagao Toshihiro 地方独立行政法人大阪産業技術研究所, 森之宮センター, 研究室長 (30416309)
• Co-Investigator(Kenkyū-buntansha): 田中 重光 地方独立行政法人大阪産業技術研究所, 生物・生活材料研究部, 研究員 (20509822)
• Research Collaborator: KISHIMOTO Noriaki 近畿大学, 農学研究院応用生物科学科, 教授 ; KURATA Atsushi 近畿大学, 農学研究院応用生物科学科, 准教授
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: Staphylococcus aureus / S. epidermidis / アトピー性皮膚炎 / パルミトレイン酸 / 皮脂 / 抗菌活性 / 菌叢 / スキンマイクロバイオーム

Outline of Final Research Achievements

Skin microbiota is concerned in several diseases and healthy condition, we thus focus on Staphylococcus aureus and S. epidermidis.
Aeromonas hydrophila converted 9cis-C18:1 to 7cis-C16:1 and 5cis-C14:1. 7cis-C16:1 showed selective anti-bacterial activity which suppressed growth of S. aureus and did not suppress growth of S. epidermidis. So, we attempted purification of 7cis-C16:1. Purification of 7cis-C16:1 was not succeeded through enzymatic process, but ODS column was effective for purification of 7cis-C16:1 (86% purity).

Free Research Field

応用微生物学、脂質工学、酵素工学