Molecular design and development of novel transition-state analogue based on reaction mechanism of hen-egg white lysozyme

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450147
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Fukushima National College of Technology
• Principal Investigator: Ogata Makoto 福島工業高等専門学校, その他部局等, 准教授 (10532666)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: リゾチーム / 遷移状態アナログ / キチンオリゴ糖 / オリゴ糖 / 阻害剤 / 酵素

Outline of Final Research Achievements

In this study, molecular design and synthesis of two transition-state analogue as inhibitors against hen-egg white lysozyme (HEWL). Furthermore, we investigated the catalytic mechanism of HEWL using these inhibitors. Next, it proved that the design principle of this inhibitor can also be applied to other polysaccharide-hydrolyzing enzyme. In addition, it was revealed that a part of the resulting oligosaccharide derivatives this time can be used as a substrate for enzyme activity measurement.

Free Research Field

生物有機化学