Molecular analysis of inhibition mechanisms of feline infectious peritonitis virus replication by cyclosporin.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450413
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: TANAKA YOSHIKAZU 日本獣医生命科学大学, 獣医学部, 准教授 (50291159)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ウイルス感染症 / ネコ伝染性腹膜炎 / ネココロナウイルス / サイクロスポリン / シクロフィリン

Outline of Final Research Achievements

Feline infectious peritonitis (FIP) is a viral infectious disease and lethal. We previously found that cyclosporin A (CsA) inhibits FIP virus (FIPV) replication in vitro and in vivo. We made cellular cyclophilin (Cyp) mutants which binds CsA less than with a wild type Cyp and transfected them into fcwf-4 cells. Consequently, these mutants suppressed FIPV replication less than those in transfected into the cells with the wild type Cyp. These findings show that CsA competitively binds the binding domain of Cyp with any cellular and/or viral factors.

Free Research Field

分子ウイルス学