2016 Fiscal Year Final Research Report
Identification and clarification of chondroitin sulfate subtype which controls skeletal muscle differentiation
| Project/Area Number |
26450444
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrative animal science
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 糖鎖 / 筋分化 / コンドロイチン硫酸 / 筋芽細胞 |
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| Outline of Final Research Achievements |
In order to clarify the control ability of skeletal muscle differentiation of chondroitin sulfate (CS), five types of CS (CS-A, B, C, D and E) were added to myoblast C2C12 which can reproduce skeletal muscle differentiation process, and differentiation was carried out for a certain period of time. The value of myotube fusion index (FI) calculated from the number of nuclei in the myotubes was used as an index of muscle differentiation. As a result, all CS subtypes used in this reserch suppressed myotube formation, among which CS-E showed remarkable suppression. Moreover, myotube FI decreased concentration-dependent manner. Conversely, knocking down the decorin of CS proteoglycan of C2C12, the muscular differentiation promoted in reverse. From the above, it has clarified that CS have a strong potential to regulate the muscle differentiation.
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| Free Research Field |
糖鎖生物学
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