Acceleration an selection of somatic nuclear reprogramming based on nuclear remodelling

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450459
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Kindai University
• Principal Investigator: TANI Tetsuya 近畿大学, 農学部, 講師 (70319763)
• Co-Investigator(Renkei-kenkyūsha): KASHIWAZAKI Naomi 麻布大学, 獣医学部, 教授 (90298232) ; ITO Junya 麻布大学, 獣医学部, 准教授 (30454143) ; FUKUDA Tomokazu 岩手大学, 理工学部, 教授 (40321640) ; FUKUHARA Takeshi 順天堂大学, 大学院医学研究科, 准教授 (20359673)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: リプログラミング / iPS細胞 / 核移植 / クローン

Outline of Final Research Achievements

Studies of nuclear reprogramming in somatic cell nuclear transfer (SCNT) and induced pluripotent stem cells (iPSCs) are good model for understanding for cell differentiation and dedifferentiation under epigenetic regulation. Efficiency of iPSCs reprogramming dramatically improved by fusion of specific amino acid transactivation domain and useful for vole and chickene. Reprogramming based on oocyte by SCNT does not required MAPK activity and improved clone embryo development by H3K9 specific demethylase Kdm4d overexpression in oocyte or somatic cell.

Free Research Field

発生工学