2017 Fiscal Year Final Research Report
Dynamics of actin cytoskeleton during mammalian spermatogenesis
| Project/Area Number |
26450499
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | マウス / 精子形成 / 受精 / 選択的スプライシング / 先体形成 |
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| Outline of Final Research Achievements |
Mammalian sperm possess a Golgi-derived exocytotic organelle, the acrosome, located on the apical region of head. We investigated genetically modified mice of acrosome protein ACRBP-W and its pre-mRNA alternative splicing variant ACRBP-V5. Consequently, the major function of ACRBP-W is to retain the inactive status of sperm serine-protease Acrosin until acrosomal exocytosis. ACRBP-V5 is involved in the formation and configuration of acrosomal granule during early spermiogenesis.Moreover, it was found that actin cytoskeleton of round spermatid was disrupted by the loss of ACRBP-V5. To observe in detail, we constructed transgenic mouse carrying the Lifeact probe which specifically recognized actin.
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| Free Research Field |
農学
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